u138 human glioma cell line  (ATCC)

Journal: Oncotarget

Article Title: Role of the P2X7 receptor in in vitro and in vivo glioma tumor growth

doi: 10.18632/oncotarget.27106

Figure Lengend Snippet: ( A ) P2X7R mRNA expression was measured by qPCR. Human U138-wt cells were used for comparison. Data are averages ± SD from at least three similar experiments analyzed by Student’s t -test. p <0.05. * Significantly different from U138-wt. ( B ) Representative trace showing intracellular calcium increase following stimulation with 100 μM BzATP in presence or absence of 25 μM A740003. ( C ) Representative trace showing ethidium bromide uptake triggered by stimulation with 200 μM BzATP. Traces are representative of at least three similar obtained in different occasions.

Article Snippet: The U138 human glioma cell line was obtained from Cell Lines Service (Eppelheim, Germany), mouse GL261 glioma cell line was a generous gift from Doctor Steve Lacroix (Department of Anatomy and Physiology, Université Laval, Quebec, Canada) and A172, T98G, M059J and U87-MG human glioma cell lines were obtained from American Type Culture Collection (ATCC).

Techniques: Expressing

Journal: Oncotarget

Article Title: Role of the P2X7 receptor in in vitro and in vivo glioma tumor growth

doi: 10.18632/oncotarget.27106

Figure Lengend Snippet: P2X7R mRNA expression was determined in transfected cells by ( A ) RT-PCR and ( B ) qPCR. P2X7R protein expression level was measured by Western blot ( C ) and flow cytometry ( D ). Representative traces ( E ) and average ( F ) intracellular Ca 2+ rise following stimulation with 200 μM BzATP. ( G ) Representative trace showing the effect of 10 μM A740003 on the intracellular calcium rise triggered by 200 μM BzATP. ( H ) Representative trace showing the effect of extracellular Ca 2+ removal on the BzATP-triggered intracellular Ca 2+ rise. ( I ) Representative trace showing 200 μM BzATP-induced ethidium bromide uptake. ( J ) Representative trace showing intracellular Ca 2+ increase and ( K ) ethidium bromide uptake following stimulation with 200 μM BzATP. Data were analyzed by ANOVA followed by Tukey test. *Significantly different from U138-wt and # significantly different from the U138-P2X7 polyclone ( p < 0.05). All experiments were carried out at least three times, except flow cytometry ( n = 1).

Article Snippet: The U138 human glioma cell line was obtained from Cell Lines Service (Eppelheim, Germany), mouse GL261 glioma cell line was a generous gift from Doctor Steve Lacroix (Department of Anatomy and Physiology, Université Laval, Quebec, Canada) and A172, T98G, M059J and U87-MG human glioma cell lines were obtained from American Type Culture Collection (ATCC).

Techniques: Expressing, Transfection, Reverse Transcription Polymerase Chain Reaction, Western Blot, Flow Cytometry

Journal: Oncotarget

Article Title: Role of the P2X7 receptor in in vitro and in vivo glioma tumor growth

doi: 10.18632/oncotarget.27106

Figure Lengend Snippet: Cell proliferation was analyzed in U138-wt cells, U138-P2X7 polyclone and U138-P2X7 clone incubated for 24 h, 48 h and 72 h in DMEM containing 1 g/L glucose (low glucose) under the following conditions: ( A ) 15% FBS DMEM; ( B ) FBS-free DMEM; ( C ) absence or presence of 10 μM A740003 in FBS-free DMEM; ( D ) in the presence of 100 μM BzATP in FBS-free DMEM; ( E ) in the presence of 10 U/mL apyrase in FBS-free DMEM. In panel F the effect of A740003 (30 μM) in GL261 cultured in 15% FBS DMEM is shown. Data were analyzed by ANOVA followed by Tukey test ( p < 0.05). All experiments were carried out at least three times.

Article Snippet: The U138 human glioma cell line was obtained from Cell Lines Service (Eppelheim, Germany), mouse GL261 glioma cell line was a generous gift from Doctor Steve Lacroix (Department of Anatomy and Physiology, Université Laval, Quebec, Canada) and A172, T98G, M059J and U87-MG human glioma cell lines were obtained from American Type Culture Collection (ATCC).

Techniques: Incubation, Cell Culture

Journal: Oncotarget

Article Title: Role of the P2X7 receptor in in vitro and in vivo glioma tumor growth

doi: 10.18632/oncotarget.27106

Figure Lengend Snippet: Cell proliferation was analyzed in ( A ) U138-wt, ( B ) U138-P2X7 polyclone and ( C ) U138-P2X7 clone cells incubated in 15% FBS-supplemented DMEM containing 4.5 g/L glucose (high glucose) for 24 h, 48 h and 72 h in the presence or absence of A740003 (10 μM). Data were analyzed by Student’s t -test. * Significantly different from DMSO ( p < 0.05). ( D ) Cell adhesion was evaluated as described in Materials and Methods. * Significantly different from U138-wt and # U138-P2X7 polyclone ( p < 0.05) by ANOVA followed by Tukey test. ( E ) Adhesion of U138-wt and U138-mock cells. Data were analyzed by Student’s t - test. ( F ) Effect of 24 h incubation in the presence of 3 mM ATP on cell viability. *Significantly different from control groups ( p < 0.05 by ANOVA). All experiments were carried out at least three times.

Article Snippet: The U138 human glioma cell line was obtained from Cell Lines Service (Eppelheim, Germany), mouse GL261 glioma cell line was a generous gift from Doctor Steve Lacroix (Department of Anatomy and Physiology, Université Laval, Quebec, Canada) and A172, T98G, M059J and U87-MG human glioma cell lines were obtained from American Type Culture Collection (ATCC).

Techniques: Incubation

Journal: Oncotarget

Article Title: Role of the P2X7 receptor in in vitro and in vivo glioma tumor growth

doi: 10.18632/oncotarget.27106

Figure Lengend Snippet: Male athymic mice were subcutaneously inoculated with ( A ) 1.4 × 10 6 or ( B ) 3.0x10 6 U138-wt, U138-P2X7 polyclone or U138-P2X7 clone cells. Tumor volume was either measured in excised tumors on p.i. day 15, or in vivo measured by caliper. Values are mean ± S.E.M. Data were analyzed by ANOVA followed by Tukey test *Significantly different from U138-wt and # significantly different from the U138-P2X7 polyclone ( p < 0.05) ( n = 3–6 animals per group). GL261 cells were subcutaneously inoculated in mice and the 30 μM A740003 was intramass-administered. ( C ) Size of in vivo tumors. Values are shown as mean ± S.E.M. Data were analyzed by ANOVA followed by Tukey test. *Significantly different from DMSO ( n = 4–5 animals per group). ( D ) The tumor volume of excised tumors at day 15 post-inoculum (4 animals per group). The values are presented as mean ± S.E.M. Data were analyzed by Student’s t - test.

Article Snippet: The U138 human glioma cell line was obtained from Cell Lines Service (Eppelheim, Germany), mouse GL261 glioma cell line was a generous gift from Doctor Steve Lacroix (Department of Anatomy and Physiology, Université Laval, Quebec, Canada) and A172, T98G, M059J and U87-MG human glioma cell lines were obtained from American Type Culture Collection (ATCC).

Techniques: In Vivo